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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMA3 Recombinant antibody

LAMA3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230443C1

Cat no : 83210-2-RR

Synonyms

LAMNA, Laminin subunit alpha 3, lama3a, E170, BM600

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMA3 Recombinant antibody (83210-2-RR)

    WB analysis using 83210-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using LAMA3 Recombinant antibody (83210-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NCI-H1299 cells, HaCaT cells, A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83210-2-RR targets LAMA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name laminin, alpha 3
Calculated molecular weight 367 kDa
Observed molecular weight260 kDa
GenBank accession numberNM_000227
Gene symbol LAMA3
Gene ID (NCBI) 3909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)

Protocols

Product Specific Protocols
WB protocol for LAMA3 antibody 83210-2-RRDownload protocol
IHC protocol for LAMA3 antibody 83210-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols