LAMC1 Monoclonal antibody

Name: LAMC1 Monoclonal antibody
Brand: Proteintech
SKU: 67706-1-Ig

LAMC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (3)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1G2G4

Review Product

Cat no : 67706-1-Ig

Synonyms

LAMB2, LAMC1, Laminin 1 subunit gamma, Laminin 10 subunit gamma, Laminin 11 subunit gamma, Laminin 2 subunit gamma, Laminin 3 subunit gamma, Laminin 4 subunit gamma, Laminin 6 subunit gamma, Laminin 7 subunit gamma, Laminin 8 subunit gamma, Laminin 9 subunit gamma, Laminin B2 chain, Laminin subunit gamma 1, S LAM gamma, S laminin subunit gamma

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis using 67706-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 67706-1-Ig
human placenta tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 67706-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat rectum using 67706-1-Ig
rat rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of pig rectum using 67706-1-Ig
pig rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 67706-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LAMC1 antibody (67706-1-Ig, Clone: 1G2G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Cat No. 67706-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human placenta tissue, LNCaP cells, rat rectum tissue, pig rectum tissue, HepG2 cells, JAR cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

67706-1-Ig targets LAMC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity	Human, Pig, Rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	LAMC1 fusion protein Ag14674 相同性解析による交差性が予測される生物種
Full Name	laminin, gamma 1 (formerly LAMB2)
Calculated molecular weight	178 kDa
Observed molecular weight	200-220 kDa
GenBank accession number	BC015586
Gene symbol	LAMC1
Gene ID (NCBI)	3915
RRID	AB_2882897
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for LAMC1 antibody 67706-1-Ig	Download protocol
IF protocol for LAMC1 antibody 67706-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Neuroscience Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety. Authors - Dan Liu View Article
human	WB	Rheumatology (Oxford) The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies Authors - Yongxin Mo View Article
rat,mouse	WB,IF	Exp Neurol LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage Authors - Qiaowei Wu View Article
human	WB	J Transl Med HIF-1α-mediated LAMC1 overexpression is an unfavorable predictor of prognosis for glioma patients: evidence from pan-cancer analysis and validation experiments Authors - Jianrong Bai View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMC1 Monoclonal antibody

LAMC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 67706-1-Ig

WB analysis of human placenta using 67706-1-Ig

WB analysis of LNCaP using 67706-1-Ig

WB analysis of rat rectum using 67706-1-Ig

WB analysis of pig rectum using 67706-1-Ig

IF Staining of HeLa using 67706-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.

The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies

LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage

HIF-1α-mediated LAMC1 overexpression is an unfavorable predictor of prognosis for glioma patients: evidence from pan-cancer analysis and validation experiments