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LAMP3 Polyclonal antibody

Name: LAMP3 Polyclonal antibody
Brand: Proteintech
SKU: 12632-1-AP
Rating: 4.00 (1 reviews)

LAMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 12632-1-AP

Synonyms

CD208, LAMP 3, LAMP, DC-lysosome-associated membrane glycoprotein, DCLAMP

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 12632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12632-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12632-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12632-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12632-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 12632-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 12632-1-AP
IP result of anti-LAMP3 (IP:12632-1-AP, 4ug; Detection:12632-1-AP 1:500) with A549 cells lysate 2800ug.
IHC staining of human lung using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12632-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 12632-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAMP3 antibody (12632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12632-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, mouse thymus tissue, rat lung tissue, A549 cells, mouse lung tissue, HEK-293T cells, Raji cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung tissue, human placenta tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 4 publications below
IF	See 11 publications below

Product Information

12632-1-AP targets LAMP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LAMP3 fusion protein Ag3325 相同性解析による交差性が予測される生物種
Full Name	lysosomal-associated membrane protein 3
Calculated molecular weight	416 aa, 44 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC032940
Gene symbol	LAMP3
Gene ID (NCBI)	27074
RRID	AB_2076629
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Protocols

Product Specific Protocols
WB protocol for LAMP3 antibody 12632-1-AP	Download protocol
IHC protocol for LAMP3 antibody 12632-1-AP	Download protocol
IF protocol for LAMP3 antibody 12632-1-AP	Download protocol
IP protocol for LAMP3 antibody 12632-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Med Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Authors - Yingxiao Shi View Article
human	IF	Arthritis Rheumatol LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands Authors - Hiroyuki Nakamura View Article
monkey	WB	Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Authors - Xianding Sun View Article
human	WB,IF	J Clin Invest Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. Authors - Ying-Qian Mo View Article
human	WB,IF	Autophagy LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization. Authors - Tsutomu Tanaka View Article
mouse	IF	Cell Prolif Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease. Authors - Xiaofan Wang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Ying (Verified Customer) (08-19-2019)

very good antibody

Applications: Western Blot,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: mouse lung tissues

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMP3 Polyclonal antibody

LAMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 12632-1-AP

WB analysis of A549 using 12632-1-AP

WB analysis of mouse lung using 12632-1-AP

WB analysis of mouse thymus using 12632-1-AP

WB analysis of A375 using 12632-1-AP

WB analysis of rat lung using 12632-1-AP

IP experiment of A549 using 12632-1-AP

IHC staining of human lung using 12632-1-AP

IHC staining of human lung using 12632-1-AP

IHC staining of human placenta using 12632-1-AP

IHC staining of human placenta using 12632-1-AP

IHC staining of human testis using 12632-1-AP

IHC staining of human testis using 12632-1-AP

IF Staining of A549 using 12632-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.

LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.

Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.

Reviews

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