- Featured Product
- KD/KO Validated
LAMP3 Polyclonal antibody
LAMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12632-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, mouse thymus tissue, rat lung tissue, A549 cells, mouse lung tissue, HEK-293T cells, Raji cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human lung tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 11 publications below |
IHC | See 4 publications below |
IF | See 10 publications below |
Product Information
12632-1-AP targets LAMP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LAMP3 fusion protein Ag3325 相同性解析による交差性が予測される生物種 |
Full Name | lysosomal-associated membrane protein 3 |
Calculated molecular weight | 416 aa, 44 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC032940 |
Gene symbol | LAMP3 |
Gene ID (NCBI) | 27074 |
RRID | AB_2076629 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LAMP3 antibody 12632-1-AP | Download protocol |
IHC protocol for LAMP3 antibody 12632-1-AP | Download protocol |
IF protocol for LAMP3 antibody 12632-1-AP | Download protocol |
IP protocol for LAMP3 antibody 12632-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Med Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. | ||
Arthritis Rheumatol LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands | ||
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. | ||
J Clin Invest Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. | ||
Autophagy LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization. | ||
Cell Prolif Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (08-19-2019) | very good antibody
|