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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMP5 Recombinant antibody (82992-2-RR)

    WB analysis using 82992-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LAMP5 Recombinant antibody (82992-2-RR)

    WB analysis using 82992-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using LAMP5 Recombinant antibody (82992-2-RR)

    IF Staining of SH-SY5Y using 82992-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using LAMP5 Recombinant antibody (82992-2-RR)

    IF Staining of Neuro-2a using 82992-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using LAMP5 Recombinant antibody (82992-2-RR)

    FC experiment of SH-SY5Y using 82992-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug LAMP5 Recombinant antibody (82992-2-RR, Clone:230102F12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82992-2-RR

    Affinity and Kinetic Characterization of 82992-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82992-2-RR against Human LAMP5 were performed. The affinity constant is 0.247 nM.

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LAMP5 Recombinant antibody

LAMP5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82992-2-RR
Clone No.230102F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

C20orf103, LAMP-5, Brain-associated LAMP-like protein, Brain and dendritic cell-associated LAMP, BAD-LAMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, U-937 cells
Positive IF/ICC detected inNeuro-2a cells, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82992-2-RR targets LAMP5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LAMP5 fusion protein Ag33894 相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 103
Observed molecular weight 31-35 kDa
GenBank accession numberBC050727
Gene Symbol LAMP5
Gene ID (NCBI) 24141
RRIDAB_3670740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UJQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP5 (Lysosome-associated membrane glycoprotein 5) is also named as BAD-LAMP and C20orf103. BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells (PMID: 21642595). LAMP5 may be one of the key genes in the development of Multiple myeloma (MM) as well as its recurrence  (PMID: 37033323). LAMP5 is a mammalian ortholog of the Caenorhabditis elegans protein, UNC-46, which functions as a sorting factor to localize the vesicular GABA transporter UNC-47 to synaptic vesicles (PMID: 30867010). It was localized exclusively in inhibitory synaptic terminals (PMID: 30867010).

Protocols

Product Specific Protocols
WB protocol for LAMP5 antibody 82992-2-RRDownload protocol
IF protocol for LAMP5 antibody 82992-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols