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KD/KO Validated

LAMR1/RPSA Polyclonal antibody

Name: LAMR1/RPSA Polyclonal antibody
Brand: Proteintech
SKU: 14533-1-AP

LAMR1/RPSA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14533-1-AP

Synonyms

LAMR1,RPSA, RPSA, Colon carcinoma laminin-binding protein, 67LR, 67 kDa laminin receptor

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 14533-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1/RPSA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14533-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14533-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 14533-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14533-1-AP (LAMR1/RPSA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14533-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LAMR1/RPSA antibody (14533-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 14533-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human LAMR1/RPSA (14533-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 14533-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, mouse liver tissue, mouse colon tissue, HeLa cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

14533-1-AP targets LAMR1/RPSA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LAMR1/RPSA fusion protein Ag6033 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein SA
Calculated molecular weight	33 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC050688
Gene symbol	RPSA
Gene ID (NCBI)	3921
RRID	AB_2182528
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.

Protocols

Product Specific Protocols
WB protocol for LAMR1/RPSA antibody 14533-1-AP	Download protocol
IHC protocol for LAMR1/RPSA antibody 14533-1-AP	Download protocol
IF protocol for LAMR1/RPSA antibody 14533-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	Neuron Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Authors - Roberta Cagnetta View Article
mouse	WB	Arterioscler Thromb Vasc Biol β1-Integrin Is Essential for Vasoregulation and Smooth Muscle Survival In Vivo. Authors - Turlo Kirsten A KA View Article
human	WB	Virulence LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination. Authors - Dingwen Hu KD Validated View Article
mouse	WB	Hum Gene Ther Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor. Authors - Hao Zhang View Article
mouse	WB	Sci Rep Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor. Authors - Xue Yan View Article
human,mouse	WB,IHC	Food Funct Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor. Authors - Lingli Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMR1/RPSA Polyclonal antibody

LAMR1/RPSA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 14533-1-AP

WB analysis of mouse liver using 14533-1-AP

WB analysis of mouse colon using 14533-1-AP

IHC staining of human stomach using 14533-1-AP

IF Staining of HepG2 using 14533-1-AP

FC experiment of HeLa using 14533-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

β1-Integrin Is Essential for Vasoregulation and Smooth Muscle Survival In Vivo.

LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.

Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.

Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor.

Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.