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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMR1/RPSA Monoclonal antibody (67324-1-Ig)

    WB analysis using 67324-1-Ig (same clone as 67324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LAMR1/RPSA Monoclonal antibody (67324-1-Ig)

    IHC staining of human lung cancer using 67324-1-Ig (same clone as 67324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LAMR1/RPSA Monoclonal antibody (67324-1-Ig)

    IHC staining of human lung cancer using 67324-1-Ig (same clone as 67324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LAMR1/RPSA Monoclonal antibody (67324-1-Ig)

    IF Staining of HepG2 using 67324-1-Ig (same clone as 67324-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1/RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

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LAMR1/RPSA Monoclonal antibody, PBS Only

LAMR1/RPSA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67324-1-PBS
Clone No.2A9A1

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67324-1-PBS targets LAMR1/RPSA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen LAMR1/RPSA fusion protein Ag6190 相同性解析による交差性が予測される生物種
Full Name ribosomal protein SA
Calculated molecular weight 33 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC050688
Gene Symbol RPSA
Gene ID (NCBI) 3921
RRIDAB_2882584
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP08865
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.