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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMR1/RPSA Recombinant antibody

LAMR1/RPSA Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240461G11

Cat no : 83495-7-RR

Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMR1/RPSA Recombinant antibody (83495-7-RR)

    WB analysis using 83495-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83495-7-RR (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LAMR1/RPSA Recombinant antibody (83495-7-RR)

    IF Staining of HepG2 using 83495-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LAMR1/RPSA antibody (83495-7-RR, Clone: 240461G11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of MCF-7 cells using LAMR1/RPSA Recombinant antibody (83495-7-RR)

    FC experiment of MCF-7 using 83495-7-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LAMR1/RPSA Recombinant antibody (83495-7-RR, Clone:240461G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83495-7-RR

    Affinity and Kinetic Characterization of 83495-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83495-7-RR against Human LAMR1,RPSA were performed. The affinity constant is 1.14 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, A549 cells, COLO 320 cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83495-7-RR is LAMR1/RPSA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LAMR1/RPSA fusion protein Ag6033 相同性解析による交差性が予測される生物種
Full Name ribosomal protein SA
Calculated molecular weight 33 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC050688
Gene symbol RPSA
Gene ID (NCBI) 3921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.

Protocols

Product Specific Protocols
WB protocol for LAMR1/RPSA antibody 83495-7-RRDownload protocol
IF protocol for LAMR1/RPSA antibody 83495-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols