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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LANCL1 Monoclonal antibody, PBS Only

LANCL1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat , pig

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H6E8

Cat no : 68160-1-PBS

Synonyms

GPR69A, LanC like protein 1, LANCL1, p40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LANCL1 Monoclonal antibody (68160-1-Ig)

    WB analysis using 68160-1-Ig (same clone as 68160-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using LANCL1 Monoclonal antibody (68160-1-Ig)

    IP experiment of mouse brain using 68160-1-Ig (same clone as 68160-1-PBS)

    IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using LANCL1 Monoclonal antibody (68160-1-Ig)

    IF Staining of U-251 using 68160-1-Ig (same clone as 68160-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68160-1-PBS targets LANCL1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LANCL1 fusion protein Ag32665 相同性解析による交差性が予測される生物種
Full Name LanC lantibiotic synthetase component C-like 1 (bacterial)
Calculated molecular weight 399 aa, 45 kDa
Observed molecular weight40 kDa
GenBank accession numberBC028685
Gene symbol LANCL1
Gene ID (NCBI) 10314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.