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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using LAP3 Monoclonal antibody (66417-1-Ig)

    WB analysis of NIH/3T3 using 66417-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using LAP3 Monoclonal antibody (66417-1-Ig)

    WB analysis of C2C12 using 66417-1-Ig

    C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LAP3 Monoclonal antibody (66417-1-Ig)

    WB analysis of HeLa using 66417-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LAP3 Monoclonal antibody (66417-1-Ig)

    WB analysis of HepG2 using 66417-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using LAP3 Monoclonal antibody (66417-1-Ig)

    IHC staining of human lung using 66417-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using LAP3 Monoclonal antibody (66417-1-Ig)

    IHC staining of human lung using 66417-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LAP3 Monoclonal antibody (66417-1-Ig)

    IF Staining of HeLa using 66417-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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LAP3 Monoclonal antibody

LAP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66417-1-Ig
Clone No.1B11B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, C2C12 cell, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66417-1-Ig targets LAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LAP3 fusion protein Ag6300 相同性解析による交差性が予測される生物種
Full Name leucine aminopeptidase 3
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC065564
Gene Symbol LAP3
Gene ID (NCBI) 51056
RRIDAB_2881789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28838
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.

Protocols

Product Specific Protocols
WB protocol for LAP3 antibody 66417-1-IgDownload protocol
IHC protocol for LAP3 antibody 66417-1-IgDownload protocol
IF protocol for LAP3 antibody 66417-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols