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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAPTM4A Polyclonal antibody

LAPTM4A Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30627-1-AP

Synonyms

HUMORF13, KIAA0108, LAPTM4, LAPTM4A, MBNT, Mtrp

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAPTM4A Polyclonal antibody (30627-1-AP)

    WB analysis using 30627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30627-1-AP (LAPTM4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LAPTM4A Polyclonal antibody (30627-1-AP)

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LAPTM4A Polyclonal antibody (30627-1-AP)

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30627-1-AP targets LAPTM4A in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAPTM4A fusion protein Ag6501 相同性解析による交差性が予測される生物種
Full Name lysosomal protein transmembrane 4 alpha
Calculated molecular weight 27 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000421
Gene symbol LAPTM4A
Gene ID (NCBI) 9741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAPTM4A is an intracellular membrane protein that regulates the function of organic cation transporter-2 (OCT2) through direct interaction. LAPTM4A can also form a complex with A4GALT in the Golgi and shows at about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for LAPTM4A antibody 30627-1-APDownload protocol
IF protocol for LAPTM4A antibody 30627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols