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  • Featured Product
  • KD/KO Validated

LARP1 Polyclonal antibody

LARP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, drosophila

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13708-1-AP

Synonyms

LARP, La-related protein 1, La related protein 1, KIAA0731

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HEK-293T using 13708-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HeLa using 13708-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HepG2 using 13708-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse heart using 13708-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse testis using 13708-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse brain using 13708-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary cancer using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13708-1-AP (LARP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary cancer using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13708-1-AP (LARP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary tumor using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary tumor using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human liver cancer using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LARP1 Polyclonal antibody (13708-1-AP)

    IF Staining of HepG2 using 13708-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (13708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse testis tissue, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13708-1-AP targets LARP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, drosophila samples.

Tested Reactivity human, mouse, rat, drosophila
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARP1 fusion protein Ag4645 相同性解析による交差性が予測される生物種
Full Name La ribonucleoprotein domain family, member 1
Calculated molecular weight 1096 aa, 124 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC033856
Gene symbol LARP1
Gene ID (NCBI) 23367
RRIDAB_2296670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LARP1 antibody 13708-1-APDownload protocol
IHC protocol for LARP1 antibody 13708-1-APDownload protocol
IF protocol for LARP1 antibody 13708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.

Authors - Pedro Fuentes
  • KO Validated
View Article
humanWB

Mol Cell Proteomics

A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications

Authors - Yuan-Qin Min
View Article
mouseWB

Mol Autism

Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.

Authors - Annie Hien
View Article