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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LARP2 Recombinant antibody

LARP2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242486E9

Cat no : 84898-3-RR

Synonyms

LARP1B, La-related protein 2, La-related protein 1B, La related protein 2, La related protein 1B

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using LARP2 Recombinant antibody (84898-3-RR)

    IHC staining of mouse testis using 84898-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84898-3-RR (LARP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LARP2 Recombinant antibody (84898-3-RR)

    IHC staining of mouse testis using 84898-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84898-3-RR (LARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LARP2 Recombinant antibody (84898-3-RR)

    IF Staining of U2OS using 84898-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using LARP2 antibody (84898-3-RR, Clone: 242486E9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using LARP2 Recombinant antibody (84898-3-RR)

    FC experiment of HeLa using 84898-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LARP2 Recombinant antibody (84898-3-RR, Clone:242486E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84898-3-RR

    Affinity and Kinetic Characterization of 84898-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84898-3-RR against Human LARP2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84898-3-RR targets LARP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LARP2 fusion protein Ag11043 相同性解析による交差性が予測される生物種
Full Name La ribonucleoprotein domain family, member 2
Calculated molecular weight 201 aa, 24 kDa
GenBank accession numberBC030516
Gene symbol LARP2
Gene ID (NCBI) 55132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for LARP2 antibody 84898-3-RRDownload protocol
IF protocol for LARP2 antibody 84898-3-RRDownload protocol
FC protocol for LARP2 antibody 84898-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols