Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
16010-1-AP targets LAS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LAS1L fusion protein Ag8732 相同性解析による交差性が予測される生物種 |
| Full Name | LAS1-like (S. cerevisiae) |
| Calculated molecular weight | 734 aa, 83 kDa |
| Observed molecular weight | 83 kDa, 70-75 kDa |
| GenBank accession number | BC014545 |
| Gene Symbol | LAS1L |
| Gene ID (NCBI) | 81887 |
| RRID | AB_2132810 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LAS1L antibody 16010-1-AP | Download protocol |
| IHC protocol for LAS1L antibody 16010-1-AP | Download protocol |
| IF protocol for LAS1L antibody 16010-1-AP | Download protocol |
| IP protocol for LAS1L antibody 16010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Breast Cancer Res Treat Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34 | ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis |