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  • Featured Product
  • KD/KO Validated

LASP1 Monoclonal antibody

LASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B6

Cat no : 68080-1-Ig

Synonyms

Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis using 68080-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis using 68080-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis of human peripheral blood platelets using 68080-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human liver cancer using 68080-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human liver cancer using 68080-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human breast cancer using 68080-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human breast cancer using 68080-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LASP1 Monoclonal antibody (68080-1-Ig)

    IF Staining of A549 using 68080-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HCT 116 cells, human peripheral blood platelets, PC-3 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68080-1-Ig targets LASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LASP1 fusion protein Ag18101 相同性解析による交差性が予測される生物種
Full Name LIM and SH3 protein 1
Calculated molecular weight 30 kDa
Observed molecular weight35-38 kDa
GenBank accession numberBC012460
Gene symbol LASP1
Gene ID (NCBI) 3927
RRIDAB_2918817
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

Protocols

Product Specific Protocols
WB protocol for LASP1 antibody 68080-1-IgDownload protocol
IHC protocol for LASP1 antibody 68080-1-IgDownload protocol
IF protocol for LASP1 antibody 68080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs

Authors - Chong Yang
  • KD Validated
View Article