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  • Featured Product
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LASS2 Polyclonal antibody

LASS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30183-1-AP

Synonyms

Tumor metastasis-suppressor gene 1 protein, Sphingosine N-acyltransferase CERS2, EC:2.3.1.297, EC:2.3.1.24, CerS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASS2 Polyclonal antibody (30183-1-AP)

    WB analysis using 30183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30183-1-AP (LASS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LASS2 Polyclonal antibody (30183-1-AP)

    WB analysis of HeLa using 30183-1-AP

    WB result of LASS2 antibody (30183-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASS2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using LASS2 Polyclonal antibody (30183-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30183-1-AP (LASS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using LASS2 Polyclonal antibody (30183-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30183-1-AP (LASS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30183-1-AP targets LASS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LASS2 fusion protein Ag32769 相同性解析による交差性が予測される生物種
Full Name LAG1 homolog, ceramide synthase 2
Calculated molecular weight 380 aa, 45 kDa
Observed molecular weight37 kDa
GenBank accession numberBC010032
Gene symbol LASS2
Gene ID (NCBI) 29956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

Protocols

Product Specific Protocols
WB protocol for LASS2 antibody 30183-1-APDownload protocol
IHC protocol for LASS2 antibody 30183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols