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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASS2 Recombinant antibody (84514-4-RR)

    WB analysis using 84514-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84514-4-RR (LASS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84514-4-RR

    Affinity and Kinetic Characterization of 84514-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84514-4-RR against Human LASS2 were performed. The affinity constant is 0.549 nM.

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LASS2 Recombinant antibody

LASS2 Recombinant Antibody for WB, ELISA
Cat No. 84514-4-RR
Clone No.241898E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Sphingosine N-acyltransferase CERS2, EC:2.3.1.297, EC:2.3.1.24, CerS2, Ceramide synthase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, MCF-7 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84514-4-RR targets LASS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LASS2 fusion protein Ag14151 相同性解析による交差性が予測される生物種
Full Name LAG1 homolog, ceramide synthase 2
Calculated molecular weight 380 aa, 45 kDa
Observed molecular weight40 kDa
GenBank accession numberBC010032
Gene Symbol LASS2
Gene ID (NCBI) 29956
RRIDAB_3672021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96G23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

Protocols

Product Specific Protocols
WB protocol for LASS2 antibody 84514-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols