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KD/KO Validated

LATS1 Polyclonal antibody

Name: LATS1 Polyclonal antibody
Brand: Proteintech
SKU: 17049-1-AP

LATS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17049-1-AP

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HepG2 using 17049-1-AP
WB result of LATS1 antibody (17049-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells.
WB analysis using 17049-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17049-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17049-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 17049-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 17049-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17049-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17049-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17049-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17049-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 17049-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 17049-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, SH-SY5Y cells, HT-29 cells, A549 cells, C2C12 cells
Positive IHC detected in	human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 43 publications below
IHC	See 9 publications below
IF	See 3 publications below
RIP	See 1 publications below

Product Information

17049-1-AP targets LATS1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LATS1 fusion protein Ag10709 相同性解析による交差性が予測される生物種
Full Name	LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated molecular weight	15 kDa, 127 kDa
Observed molecular weight	140-150 kDa, 120 kDa
GenBank accession number	BC015665
Gene symbol	LATS1
Gene ID (NCBI)	9113
RRID	AB_2281011
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Protocols

Product Specific Protocols
WB protocol for LATS1 antibody 17049-1-AP	Download protocol
IHC protocol for LATS1 antibody 17049-1-AP	Download protocol
IF protocol for LATS1 antibody 17049-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Res A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling. Authors - Rui-Hua Li View Article
human	WB	Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis. Authors - Shujie Chen View Article
human	WB	Theranostics CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways. Authors - Lixia Chen View Article
	IHC	JCI Insight KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline Authors - Kristin Meliambro View Article
	WB	Oncogene Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma. Authors - Yongyun Li View Article
	IHC,WB	Acta Pharmacol Sin KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury. Authors - Dan Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 17049-1-AP

WB analysis using 17049-1-AP

WB analysis of K-562 using 17049-1-AP

WB analysis of SH-SY5Y using 17049-1-AP

WB analysis using 17049-1-AP

IHC staining of human breast cancer using 17049-1-AP

IHC staining of human breast cancer using 17049-1-AP

IHC staining of human testis using 17049-1-AP

IHC staining of human testis using 17049-1-AP

IF Staining of HeLa using 17049-1-AP

IF Staining of HeLa using 17049-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling.

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.

KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury.