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LATS2-Specific Polyclonal antibody

Name: LATS2-Specific Polyclonal antibody
Brand: Proteintech
SKU: 20276-1-AP

LATS2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20276-1-AP

Synonyms

LATS2, Serine/threonine-protein kinase kpm, LATS 2, KPM, EC:2.7.11.1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 20276-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20276-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 20276-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 20276-1-AP
IP result of anti-LATS2-Specific (IP:20276-1-AP, 4ug; Detection:20276-1-AP 1:300) with HEK-293 cells lysate 2320 ug.
IHC staining of human liver cancer using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver cancer using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 20276-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20276-1-AP
Immunofluorescent analysis of HepG2 cells using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 20276-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, human heart tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human colon tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 31 publications below
IHC	See 9 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

20276-1-AP targets LATS2-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	LATS, large tumor suppressor, homolog 2 (Drosophila)
Calculated molecular weight	120 kDa
Observed molecular weight	130-150 kDa
GenBank accession number	NM_014572
Gene symbol	LATS2
Gene ID (NCBI)	26524
RRID	AB_10697657
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.

Protocols

Product Specific Protocols
WB protocol for LATS2-Specific antibody 20276-1-AP	Download protocol
IHC protocol for LATS2-Specific antibody 20276-1-AP	Download protocol
IF protocol for LATS2-Specific antibody 20276-1-AP	Download protocol
IP protocol for LATS2-Specific antibody 20276-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer Authors - Yufan Ying View Article
mouse	WB	Cell Metab Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis. Authors - Xiaobo Wang KD Validated View Article
human	WB	Cell Death Differ The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway. Authors - Tian-Sheng He KO Validated View Article
human	WB, CoIP	Clin Transl Med Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway. Authors - Jing Chen View Article
human	WB,IHC	Oxid Med Cell Longev miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway. Authors - Tangwei Wu View Article
human	WB	Oncogene TRIB3 confers radiotherapy resistance in esophageal squamous cell carcinoma by stabilizing TAZ. Authors - Sha Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LATS2-Specific Polyclonal antibody

LATS2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 20276-1-AP

WB analysis of mouse brain using 20276-1-AP

WB analysis of human heart using 20276-1-AP

IP experiment of HEK-293 using 20276-1-AP

IHC staining of human liver cancer using 20276-1-AP

IHC staining of human liver cancer using 20276-1-AP

IHC staining of human colon using 20276-1-AP

IHC staining of human colon using 20276-1-AP

IHC staining of human liver cancer using 20276-1-AP

IHC staining of mouse skeletal muscle using 20276-1-AP

IHC staining of mouse skeletal muscle using 20276-1-AP

IF Staining of HepG2 using 20276-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer

Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.

The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway.

Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway.

TRIB3 confers radiotherapy resistance in esophageal squamous cell carcinoma by stabilizing TAZ.