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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAYN Polyclonal antibody

LAYN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20535-1-AP

Synonyms

UNQ208/PRO234, Layilin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAYN Polyclonal antibody (20535-1-AP)

    WB analysis using 20535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20535-1-AP (LAYN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LAYN Polyclonal antibody (20535-1-AP)

    WB analysis of mouse brain using 20535-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20535-1-AP (LAYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAYN Polyclonal antibody (20535-1-AP)

    IHC staining of human liver cancer using 20535-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20535-1-AP (LAYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAYN Polyclonal antibody (20535-1-AP)

    IHC staining of human liver cancer using 20535-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20535-1-AP (LAYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAYN Polyclonal antibody (20535-1-AP)

    IHC staining of human liver cancer using 20535-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20535-1-AP (LAYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAYN Polyclonal antibody (20535-1-AP)

    IHC staining of human liver cancer using 20535-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20535-1-AP (LAYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LAYN Polyclonal antibody (20535-1-AP)

    IF Staining of HepG2 using 20535-1-AP

    Immunofluorescent analysis of HepG2 cells, using LAYN antibody 20535-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, NCI-H1299 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20535-1-AP targets LAYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAYN fusion protein Ag14508 相同性解析による交差性が予測される生物種
Full Name layilin
Calculated molecular weight 382 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC025407
Gene symbol LAYN
Gene ID (NCBI) 143903
RRIDAB_10696315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LAYN antibody 20535-1-APDownload protocol
IHC protocol for LAYN antibody 20535-1-APDownload protocol
IF protocol for LAYN antibody 20535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Environ Pollut

Silica nanoparticles suppressed the spermatogenesis via downregulation of miR-450b-3p by targeting Layilin in spermatocyte of mouse

Authors - Guiqing Zhou
View Article