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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LBP Polyclonal antibody

LBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11836-1-AP

Synonyms

Lipopolysaccharide-binding protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of mouse liver using 11836-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of L02 using 11836-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of Jurkat using 11836-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of A431 using 11836-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of HeLa using 11836-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of A549 using 11836-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of A549 using 11836-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of rat liver using 11836-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LBP Polyclonal antibody (11836-1-AP)

    WB analysis of A549 using 11836-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using LBP Polyclonal antibody (11836-1-AP)

    IP experiment of mouse liver using 11836-1-AP

    IP result of anti-LBP (IP:11836-1-AP, 4ug; Detection:11836-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Polyclonal antibody (11836-1-AP)

    IHC staining of human liver using 11836-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Polyclonal antibody (11836-1-AP)

    IHC staining of human liver using 11836-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using LBP Polyclonal antibody (11836-1-AP)

    IF Staining of L02 using 11836-1-AP

    Immunofluorescent analysis of L02 cells using 11836-1-AP (LBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LBP Polyclonal antibody (11836-1-AP)

    IF Staining of HepG2 using 11836-1-AP

    Immunofluorescent analysis of HepG2 cells, using LBP antibody 11836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inmouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11836-1-AP targets LBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LBP fusion protein Ag2431 相同性解析による交差性が予測される生物種
Full Name lipopolysaccharide binding protein
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 63-67 kDa
GenBank accession numberBC022256
Gene symbol LBP
Gene ID (NCBI) 3929
RRIDAB_2097479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60 kDa and 63-67 kDa(Glycosylation form).

Protocols

Product Specific Protocols
WB protocol for LBP antibody 11836-1-APDownload protocol
IHC protocol for LBP antibody 11836-1-APDownload protocol
IF protocol for LBP antibody 11836-1-APDownload protocol
IP protocol for LBP antibody 11836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Neurosurg

Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.

Authors - Nataliya Smith
View Article
humanWB

Cancer Sci

Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.

Authors - Ning Wang
View Article
ratIHC

J Biomed Mater Res A

The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.

Authors - Takafumi Kobayashi
View Article