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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LBP Monoclonal antibody (66181-1-Ig)

    WB analysis of Jurkat using 66181-1-Ig (same clone as 66181-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver cancer using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver cancer using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human kidney using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human kidney using 66181-1-Ig (same clone as 66181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

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LBP Monoclonal antibody, PBS Only

LBP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66181-1-PBS
Clone No.3A7E3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

LBP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66181-1-PBS targets LBP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LBP fusion protein Ag13485 相同性解析による交差性が予測される生物種
Full Name lipopolysaccharide binding protein
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC022256
Gene Symbol LBP
Gene ID (NCBI) 3929
RRIDAB_2881576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18428
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)