Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83427-1-RR targets LBP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | lipopolysaccharide binding protein |
| Calculated molecular weight | 53KD |
| Observed molecular weight | 63-67 kDa |
| GenBank accession number | NM_004139.4 |
| Gene Symbol | LBP |
| Gene ID (NCBI) | 3929 |
| RRID | AB_3671074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P18428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LBP antibody 83427-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |