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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LBR Monoclonal antibody, PBS Only

LBR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B7

Cat no : 68378-1-PBS

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LBR Monoclonal antibody (68378-1-Ig)

    WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LBR Monoclonal antibody (68378-1-Ig)

    WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LBR Monoclonal antibody (68378-1-Ig)

    IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LBR Monoclonal antibody (68378-1-Ig)

    IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LBR Monoclonal antibody (68378-1-Ig)

    IF Staining of HeLa using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (68378-1-Ig, Clone: 1A1B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68378-1-PBS targets LBR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LBR fusion protein Ag25601 相同性解析による交差性が予測される生物種
Full Name lamin B receptor
Calculated molecular weight 615 aa, 71 kDa
Observed molecular weight58 kDa
GenBank accession numberBC020079
Gene symbol LBR
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).