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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LBR Recombinant antibody, PBS Only

LBR Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241864H1

Cat no : 84522-6-PBS

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LBR Recombinant antibody (84522-6-RR)

    WB analysis using 84522-6-RR (same clone as 84522-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84522-6-RR (LBR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using LBR Recombinant antibody (84522-6-RR)

    FC experiment of HepG2 using 84522-6-RR (same clone as 84522-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LBR Recombinant antibody (84522-6-RR)

    FC experiment of HeLa using 84522-6-RR (same clone as 84522-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84522-6-PBS is LBR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LBR fusion protein Ag3088 相同性解析による交差性が予測される生物種
Full Name lamin B receptor
Calculated molecular weight 615 aa, 71 kDa
Observed molecular weight58 kDa
GenBank accession numberBC020079
Gene symbol LBR
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).