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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LC3 Recombinant antibody (81004-1-RR)

    WB analysis of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    WB result of LC3 antibody (81004-1-RR; 1:20000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LC3 Recombinant antibody (81004-1-RR)

    WB analysis using 81004-1-RR (same clone as 81004-1-PBS)

    Untreated and chloroquine treated HEK-293 cells, untreated and chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using LC3 Recombinant antibody (81004-1-RR)

    WB analysis of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LC3 Recombinant antibody (81004-1-RR)

    IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LC3 Recombinant antibody (81004-1-RR)

    IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3 Recombinant antibody (81004-1-RR)

    IHC staining of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3 Recombinant antibody (81004-1-RR)

    IHC staining of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LC3 Recombinant antibody (81004-1-RR)

    IF Staining of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3 antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81004-1-RR

    Affinity and Kinetic Characterization of 81004-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81004-1-RR against Human LC3 were performed. The affinity constant is 0.967 nM.

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  • Featured Product
  • KD/KO Validated

LC3 Recombinant antibody, PBS Only

LC3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81004-1-PBS
Clone No.5P12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MAP1LC3B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B, Autophagy-related ubiquitin-like modifier LC3 B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81004-1-PBS targets LC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LC3 fusion protein Ag6144 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
Observed molecular weight14-18 kDa
GenBank accession numberBC067797
Gene Symbol LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZQ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.