Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 1 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
Product Information
66139-1-Ig targets LC3A/B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse samples.
| Tested Reactivity | human, pig, mouse |
| Cited Reactivity | human, mouse, rat, pig, hamster |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein 1 light chain 3 alpha |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14 kDa, 16 kDa |
| GenBank accession number | NM_032514 |
| Gene Symbol | LC3 |
| Gene ID (NCBI) | 84557 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H492 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
Publications
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Phytomedicine Salidroside facilitates neuroprotective effects in ischemic stroke by promoting axonal sprouting through promoting autophagy | ||
Cell Death Dis Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy. | ||
Cell Prolif S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos. | ||
Mol Neurobiol Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy. | ||
Aging (Albany NY) Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs. |
