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LCK Polyclonal antibody

LCK Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12477-1-AP

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16


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Tested Applications

Positive WB detected inJurkat cells, Raji cells, Ramos cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

12477-1-AP targets LCK in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCK fusion protein Ag3176 相同性解析による交差性が予測される生物種
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene symbol LCK
Gene ID (NCBI) 3932
RRIDAB_2136316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for LCK antibody 12477-1-APDownload protocol
IP protocol for LCK antibody 12477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Ovarian Res

Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors.

Authors - Katie K Crean-Tate
mouseWB

Front Pharmacol

"Drying effect" of fructus aurantii components and the mechanism of action based on network pharmacology and in vitro pharmacodynamic validation

Authors - Jing Zhu
humanWB

J Ovarian Res

LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer

Authors - Goutam Dey
mouseWB

Nat Commun

Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function

Authors - Yuman Wang