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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LCMT1 Polyclonal antibody (30222-1-AP)

    WB analysis using 30222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30222-1-AP (LCMT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse uterus tissue using LCMT1 Polyclonal antibody (30222-1-AP)

    IHC staining of mouse uterus using 30222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 30222-1-AP (LCMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LCMT1 Polyclonal antibody

LCMT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30222-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

CGI 68, LCMT, LCMT1, PPMT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cellls, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse uterus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30222-1-AP targets LCMT1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCMT1 fusion protein Ag32038 相同性解析による交差性が予測される生物種
Full Name leucine carboxyl methyltransferase 1
Calculated molecular weight 38 kDa
Observed molecular weight 38-41 kDa
GenBank accession numberBC001214
Gene Symbol LCMT1
Gene ID (NCBI) 51451
RRIDAB_2935534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leucine carboxyl methyltransferase 1 (LCMT1) belongs to the methyltransferase superfamily (LCMT family). It methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. LCMT1 has 3 isoforms with the MW of 38 kDa, 41 kDa, and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for LCMT1 antibody 30222-1-APDownload protocol
IHC protocol for LCMT1 antibody 30222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols