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L-Plastin Polyclonal antibody
L-Plastin Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13025-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, mouse spleen tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 4 publications below |
FC | See 1 publications below |
Product Information
13025-1-AP targets L-Plastin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | L-Plastin fusion protein Ag3571 相同性解析による交差性が予測される生物種 |
Full Name | lymphocyte cytosolic protein 1 (L-plastin) |
Calculated molecular weight | 627 aa, 64 kDa |
Observed molecular weight | 64-70 kDa |
GenBank accession number | BC010271 |
Gene symbol | L-Plastin |
Gene ID (NCBI) | 3936 |
RRID | AB_2136871 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for L-Plastin antibody 13025-1-AP | Download protocol |
IHC protocol for L-Plastin antibody 13025-1-AP | Download protocol |
IP protocol for L-Plastin antibody 13025-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Signal A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity | ||
Mol Ther Nucleic Acids Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway. | ||
Cancer Biomark LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer | ||
Drug Dev Res Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway | ||
Pharm Res Transcriptome Profiling Analysis Identifies LCP1 as a Contributor for Chidamide Resistance in Gastric Cancer.
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