Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11004-1-AP targets LDB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LDB3 fusion protein Ag1417 相同性解析による交差性が予測される生物種 |
| Full Name | LIM domain binding 3 |
| Calculated molecular weight | 32/78 kDa |
| Observed molecular weight | 32 kDa, 78-80 kDa |
| GenBank accession number | BC010929 |
| Gene Symbol | LDB3 |
| Gene ID (NCBI) | 11155 |
| RRID | AB_2137049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LDB3 antibody 11004-1-AP | Download protocol |
| IHC protocol for LDB3 antibody 11004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation. | ||
Hum Mol Genet Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion. | ||
NPJ Precis Oncol Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling |