Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21799-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-231 using 21799-1-AP
WB result of LDHA antibody (21799-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells.
WB analysis of MCF-7 using 21799-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21799-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 21799-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 21799-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21799-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21799-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 21799-1-AP
IP result of anti-LDHA (IP:21799-1-AP, 4ug; Detection:21799-1-AP 1:500) with HEK-293 cells lysate 2000ug.
IHC staining of human skeletal muscle using 21799-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 21799-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 21799-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA antibody (21799-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 21799-1-AP
Immunofluorescent analysis of HepG2 cells, using LDHA antibody 21799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

LDHA Polyclonal antibody

LDHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21799-1-AP

Publications(61)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LDH-M, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (61)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, MCF-7 cells, human testis tissue, A375 cells, HepG2 cells, NIH/3T3 cells, MDA-MB-231 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:30000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 55 publications below
IHC	See 12 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

21799-1-AP targets LDHA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LDHA fusion protein Ag16703 相同性解析による交差性が予測される生物種
Full Name	lactate dehydrogenase A
Calculated molecular weight	332 aa, 37 kDa
Observed molecular weight	32-37 kDa
GenBank accession number	BC067223
Gene Symbol	LDHA
Gene ID (NCBI)	3939
RRID	AB_10858925
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00338
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).

Protocols

Product Specific Protocols
WB protocol for LDHA antibody 21799-1-AP	Download protocol
IHC protocol for LDHA antibody 21799-1-AP	Download protocol
IF protocol for LDHA antibody 21799-1-AP	Download protocol
IP protocol for LDHA antibody 21799-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell A tissue injury sensing and repair pathway distinct from host pathogen defense Authors - Siqi Liu View Article
mouse	IF	Nat Metab Time of exercise differentially impacts bone growth in mice Authors - Shaoling Yu View Article
human	WB	Sci Transl Med Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice. Authors - Biying Zhu View Article
human	WB,IF,IP	Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. Authors - Youming Zhu View Article
human	WB	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
		Cancer Res HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. Authors - Yinghui Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21799-1-AP

WB analysis of MDA-MB-231 using 21799-1-AP

WB analysis of MCF-7 using 21799-1-AP

WB analysis of HEK-293 using 21799-1-AP

WB analysis of human testis using 21799-1-AP

WB analysis of A375 using 21799-1-AP

WB analysis of HepG2 using 21799-1-AP

WB analysis of NIH/3T3 using 21799-1-AP

IP experiment of HEK-293 using 21799-1-AP

IHC staining of human skeletal muscle using 21799-1-AP

IHC staining of human skeletal muscle using 21799-1-AP

IF Staining of HepG2 using 21799-1-AP

IF Staining of HepG2 using 21799-1-AP

LDHA Polyclonal antibody

LDHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A tissue injury sensing and repair pathway distinct from host pathogen defense

Time of exercise differentially impacts bone growth in mice

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.