Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-231 using 19987-1-AP
WB result of LDHA-Specific antibody (19987-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells.
WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19987-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 19987-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 19987-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19987-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19987-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 19987-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 19987-1-AP
IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skeletal muscle using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 19987-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 19987-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19987-1-AP

Publications(258)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (258)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, MDA-MB-231 cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human heart tissue, human skeletal muscle tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 227 publications below
IHC	See 40 publications below
IF	See 14 publications below
IP	See 6 publications below
FC	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

19987-1-AP targets LDHA-Specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, chicken, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	lactate dehydrogenase A
Calculated molecular weight	37 kDa
Observed molecular weight	32-37 kDa
GenBank accession number	NM_005566
Gene Symbol	LDHA
Gene ID (NCBI)	3939
RRID	AB_10646429
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00338
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IHC protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IF protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IP protocol for LDHA-Specific antibody 19987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	WB	Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis Authors - View Article
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
human	WB	Drug Resist Updat Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression Authors - Shuai Wang KD Validated View Article
human		Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury Authors - Shuyong Wang View Article
human		Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. Authors - Di Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19987-1-AP

WB analysis of MDA-MB-231 using 19987-1-AP

WB analysis using 19987-1-AP

WB analysis of HeLa using 19987-1-AP

WB analysis using 19987-1-AP

WB analysis using 19987-1-AP

WB analysis of mouse testis using 19987-1-AP

WB analysis of rat kidney using 19987-1-AP

WB analysis of Jurkat using 19987-1-AP

WB analysis of Jurkat using 19987-1-AP

WB analysis of A431 using 19987-1-AP

IP experiment of mouse kidney using 19987-1-AP

IHC staining of human heart using 19987-1-AP

IHC staining of human heart using 19987-1-AP

IHC staining of human skeletal muscle using 19987-1-AP

IHC staining of human skeletal muscle using 19987-1-AP

IHC staining of human testis using 19987-1-AP

IHC staining of human testis using 19987-1-AP

IHC staining of human skeletal muscle using 19987-1-AP

IHC staining of human skeletal muscle using 19987-1-AP

IF Staining of HepG2 using 19987-1-AP

IF Staining of HepG2 using 19987-1-AP

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetate enables metabolic fitness and cognitive performance during sleep disruption

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.