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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-231 cells using LDHA-Specific Recombinant antibody (84198-2-RR)

    WB analysis of MDA-MB-231 using 84198-2-RR (same clone as 84198-2-PBS)

    WB result of LDHA-Specific antibody (84198-2-RR; 1:3000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LDHA-Specific Recombinant antibody (84198-2-RR)

    WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84198-2-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using LDHA-Specific Recombinant antibody (84198-2-RR)

    FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-2-RR, Clone:240885A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

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  • KD/KO Validated

LDHA-Specific Recombinant antibody, PBS Only

LDHA-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84198-2-PBS
Clone No.240885A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84198-2-PBS targets LDHA-Specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase A
Calculated molecular weight 37 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_005566
Gene Symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.