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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LDHA-Specific Recombinant antibody

LDHA-Specific Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240885C7

Cat no : 84198-3-RR

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDHA-Specific Recombinant antibody (84198-3-RR)

    WB analysis using 84198-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84198-3-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using LDHA-Specific Recombinant antibody (84198-3-RR)

    FC experiment of HepG2 using 84198-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-3-RR, Clone:240885C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84198-3-RR targets LDHA-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase A
Calculated molecular weight 37 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_005566
Gene symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 84198-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols