- Featured Product
- KD/KO Validated
LDHB-Specific Polyclonal antibody
LDHB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19988-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, HEK-293T cells, Jurkat cells, PC-3 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
19988-1-AP targets LDHB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lactate dehydrogenase B |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | NM_002300 |
| Gene symbol | LDHB |
| Gene ID (NCBI) | 3945 |
| RRID | AB_10638780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IHC protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IF protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomedicines HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine. | ||
J Nutr Sci Vitaminol (Tokyo) Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice | ||
Mol Biol Rep Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies. | ||
Am J Physiol Renal Physiol Expression of lactate dehydrogenase A and B isoforms in the mouse kidney. | ||
iScience Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma
| ||
iScience Altered neuronal lactate dehydrogenase A expression affects cognition in a sex- and age-dependent manner |
