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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDHB Monoclonal antibody (66425-1-Ig)

    WB analysis using 66425-1-Ig (same clone as 66425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LDHB Monoclonal antibody (66425-1-Ig)

    IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LDHB Monoclonal antibody (66425-1-Ig)

    IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDHB Monoclonal antibody (66425-1-Ig)

    IF Staining of HeLa using 66425-1-Ig (same clone as 66425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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LDHB Monoclonal antibody, PBS Only

LDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66425-1-PBS
Clone No.5E9G1

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66425-1-PBS targets LDHB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LDHB fusion protein Ag6605 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase B
Calculated molecular weight 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002362
Gene Symbol LDHB
Gene ID (NCBI) 3945
RRIDAB_2881796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07195
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.