Featured Product
KD/KO Validated

LDLR Polyclonal antibody

Name: LDLR Polyclonal antibody
Brand: Proteintech
SKU: 10785-1-AP

LDLR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

103

Review Product

Cat no : 10785-1-AP

Synonyms

LDL receptor, FHC, FH

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10785-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10785-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10785-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10785-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10785-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10785-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10785-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10785-1-AP
IP result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of mouse brain using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human brain using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human pancreas using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 10785-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10785-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

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Cat No. 10785-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, human brain tissue, Jurkat cells, mouse brain tissue, Raji cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human brain tissue, mouse liver tissue, human pancreas tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 95 publications below
IHC	See 3 publications below
IF	See 10 publications below
IP	See 3 publications below
FC	See 1 publications below

Product Information

10785-1-AP targets LDLR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, chicken, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LDLR fusion protein Ag1236 相同性解析による交差性が予測される生物種
Full Name	low density lipoprotein receptor
Calculated molecular weight	95 kDa
Observed molecular weight	100-160 kDa
GenBank accession number	BC014514
Gene symbol	LDLR
Gene ID (NCBI)	3949
RRID	AB_2281164
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 10785-1-AP	Download protocol
IHC protocol for LDLR antibody 10785-1-AP	Download protocol
IF protocol for LDLR antibody 10785-1-AP	Download protocol
IP protocol for LDLR antibody 10785-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Authors - Yi Fu View Article
human	WB	Cell Metab Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial. Authors - Jiang Wang View Article
human,mouse	WB,IF	Sci Transl Med Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology Authors - Su'an Tang View Article
mouse	WB,IP	J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. Authors - Shanshan Zhong KO Validated View Article
human	WB	Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Authors - Yunkai Zhu View Article
mouse	IF	Adv Healthc Mater Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer. Authors - Yi Pan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LDLR Polyclonal antibody

LDLR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10785-1-AP

WB analysis of HeLa using 10785-1-AP

WB analysis of Raji using 10785-1-AP

WB analysis of HL-60 using 10785-1-AP

WB analysis of Jurkat using 10785-1-AP

WB analysis of mouse brain using 10785-1-AP

WB analysis of human brain using 10785-1-AP

IP experiment of HeLa using 10785-1-AP

IHC staining of mouse brain using 10785-1-AP

IHC staining of mouse brain using 10785-1-AP

IHC staining of mouse liver using 10785-1-AP

IHC staining of mouse liver using 10785-1-AP

IHC staining of human colon cancer using 10785-1-AP

IHC staining of human colon cancer using 10785-1-AP

IHC staining of human brain using 10785-1-AP

IHC staining of human brain using 10785-1-AP

IHC staining of human pancreas using 10785-1-AP

IHC staining of human pancreas using 10785-1-AP

IF Staining of HeLa using 10785-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer.

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