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  • Featured Product
  • KD/KO Validated

LDLR Monoclonal antibody, PBS Only

LDLR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 66414-1-PBS

Synonyms

LDL receptor, FHC, FH, 1A2A3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LDLR Monoclonal antibody (66414-1-Ig)

    WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

    WB result of LDLR antibody (66414-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDLR transfected HeLa cells. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LDLR Monoclonal antibody (66414-1-Ig)

    WB analysis using 66414-1-Ig (same clone as 66414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using LDLR Monoclonal antibody (66414-1-Ig)

    WB analysis of HEK-293 using 66414-1-Ig (same clone as 66414-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LDLR Monoclonal antibody (66414-1-Ig)

    WB analysis of Jurkat using 66414-1-Ig (same clone as 66414-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LDLR Monoclonal antibody (66414-1-Ig)

    WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LDLR Monoclonal antibody (66414-1-Ig)

    IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LDLR Monoclonal antibody (66414-1-Ig)

    IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66414-1-PBS targets LDLR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LDLR fusion protein Ag1236 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein receptor
Calculated molecular weight 95 kDa
Observed molecular weight 120 kDa, 160 kDa
GenBank accession numberBC014514
Gene symbol LDLR
Gene ID (NCBI) 3949
RRIDAB_2881786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.