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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LDLR Recombinant antibody

LDLR Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1O11

Cat no : 82724-1-RR

Synonyms

LDL receptor, FHC, FH, 1O11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDLR Recombinant antibody (82724-1-RR)

    WB analysis using 82724-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LDLR Recombinant antibody (82724-1-RR)

    IHC staining of mouse brain using 82724-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LDLR Recombinant antibody (82724-1-RR)

    IHC staining of mouse brain using 82724-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDLR Recombinant antibody (82724-1-RR)

    IF Staining of HeLa using 82724-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82724-1-RR

    Affinity and Kinetic Characterization of 82724-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82724-1-RR targets LDLR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LDLR fusion protein Ag1236 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein receptor
Calculated molecular weight 95 kDa
Observed molecular weight140-160 kDa
GenBank accession numberBC014514
Gene symbol LDLR
Gene ID (NCBI) 3949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 82724-1-RRDownload protocol
IHC protocol for LDLR antibody 82724-1-RRDownload protocol
IF protocol for LDLR antibody 82724-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols