• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ARH Polyclonal antibody

ARH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13213-1-AP

Synonyms

ARH, ARH1, ARH2, FHCB1, FHCB2, LDLRAP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ARH Polyclonal antibody (13213-1-AP)

    WB analysis using 13213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13213-1-AP (ARH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARH Polyclonal antibody (13213-1-AP)

    WB analysis of HeLa using 13213-1-AP

    WB result of ARH antibody (13213-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARH transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ARH Polyclonal antibody (13213-1-AP)

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ARH Polyclonal antibody (13213-1-AP)

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13213-1-AP targets ARH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARH fusion protein Ag4006 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein receptor adaptor protein 1
Calculated molecular weight 308 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC029770
Gene symbol ARH
Gene ID (NCBI) 26119
RRIDAB_2135283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARH antibody 13213-1-APDownload protocol
IHC protocol for ARH antibody 13213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis

Authors - Colin T Stomberski
View Article
mouseWB

Cell Biol Toxicol

Activation of lipophagy ameliorates cadmium-induced neural tube defects via reducing low density lipoprotein cholesterol levels in mouse placentas

Authors - Yu-Feng Zhang
View Article