Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat heart tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83586-1-RR targets LEFTY1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | LEFTY1 fusion protein Ag4925 相同性解析による交差性が予測される生物種 |
| Full Name | left-right determination factor 1 |
| Calculated molecular weight | 366 aa, 41 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC027883 |
| Gene Symbol | LEFTY1 |
| Gene ID (NCBI) | 10637 |
| RRID | AB_3671200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O75610 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lefty is a member of the transforming growth factor (TGF) β superfamily, which is implicated in left-right patterning during embryogenesis. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LEFTY1 antibody 83586-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |