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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LELP1 Polyclonal antibody (32483-1-AP)

    WB analysis using 32483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32483-1-AP (LELP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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LELP1 Polyclonal antibody

LELP1 Polyclonal Antibody for WB, ELISA
Cat No. 32483-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Novel small proline-rich protein, Late cornified envelope-like proline-rich protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32483-1-AP targets LELP1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LELP1 fusion protein Ag36831 相同性解析による交差性が予測される生物種
Full Name late cornified envelope-like proline-rich 1
Calculated molecular weight11 kDa
Observed molecular weight17 kDa
GenBank accession numberBC130507
Gene Symbol LELP1
Gene ID (NCBI) 149018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5T871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Late cornified envelope-like proline-rich protein 1 (LELP1) is part of the epidermal differentiation complex (EDC), a cluster of genes that encode proteins essential for the differentiation of keratinocytes and the formation of the stratum corneum (PMID: 34112911). A single nucleotide polymorphism (SNP) in the LELP1 gene has been associated with atopic dermatitis, a chronic inflammatory skin disorder characterized by elevated levels of serum immunoglobulin E (IgE) and severe skin inflammation (PMID: 26608070).

Protocols

Product Specific Protocols
WB protocol for LELP1 antibody 32483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols