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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LEMD2 Polyclonal antibody (29406-1-AP)

    WB analysis using 29406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29406-1-AP (LEMD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using LEMD2 Polyclonal antibody (29406-1-AP)

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using LEMD2 Polyclonal antibody (29406-1-AP)

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LEMD2 Polyclonal antibody (29406-1-AP)

    IHC staining of mouse kidney using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LEMD2 Polyclonal antibody (29406-1-AP)

    IHC staining of mouse kidney using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LEMD2 Polyclonal antibody

LEMD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29406-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

dJ482C21.1, hLEM2, LEM domain containing 2, LEMD2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells, human testis tissue
Positive IHC detected inhuman colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29406-1-AP targets LEMD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LEMD2 fusion protein Ag28078 相同性解析による交差性が予測される生物種
Full Name LEM domain containing 2
Calculated molecular weight 503 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC051803
Gene Symbol LEMD2
Gene ID (NCBI) 221496
RRIDAB_2918298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LEMD2 antibody 29406-1-APDownload protocol
IHC protocol for LEMD2 antibody 29406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols