LEO1 Polyclonal antibody

Name: LEO1 Polyclonal antibody
Brand: Proteintech
SKU: 12281-1-AP

LEO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12281-1-AP

Synonyms

RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, RDL

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of mouse heart using 12281-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12281-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12281-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12281-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12281-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12281-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12281-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12281-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 12281-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 12281-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12281-1-AP (LEO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12281-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12281-1-AP (LEO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12281-1-AP
Immunofluorescent analysis of Hela cells, using LEO1 antibody 12281-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 12281-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, HeLa cells, HL-60 cells, human placenta tissue, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IP	See 1 publications below

Product Information

12281-1-AP targets LEO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LEO1 fusion protein Ag2932 相同性解析による交差性が予測される生物種
Full Name	Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight	666 aa, 75 kDa
Observed molecular weight	105 kDa
GenBank accession number	BC018147
Gene symbol	LEO1
Gene ID (NCBI)	123169
RRID	AB_10640429
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Protocols

Product Specific Protocols
WB protocol for LEO1 antibody 12281-1-AP	Download protocol
IHC protocol for LEO1 antibody 12281-1-AP	Download protocol
IF protocol for LEO1 antibody 12281-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Neurosci Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD. Authors - Lindsey D Goodman View Article
mouse	WB	Cell Death Dis UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. Authors - Gang Xiang View Article
human	WB	Sci Adv CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation Authors - Min Qiu View Article
mouse	WB,IP	Cell Inherited blood cancer predisposition through altered transcription elongation Authors - Jiawei Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LEO1 Polyclonal antibody

LEO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse heart using 12281-1-AP

WB analysis of human placenta using 12281-1-AP

WB analysis of Jurkat using 12281-1-AP

WB analysis of mouse brain using 12281-1-AP

WB analysis of HeLa using 12281-1-AP

WB analysis of mouse heart using 12281-1-AP

WB analysis of HeLa using 12281-1-AP

WB analysis of mouse skeletal muscle using 12281-1-AP

WB analysis of HL-60 using 12281-1-AP

IHC staining of human colon using 12281-1-AP

IHC staining of human colon using 12281-1-AP

IF Staining of HeLa using 12281-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD.

UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.

CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation

Inherited blood cancer predisposition through altered transcription elongation