Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HT-29 cells, A549 cells, HEK-293 cells, LNCaP cells, MCF-7 cells, Hela cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68568-1-Ig targets LEO1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | LEO1 fusion protein Ag33577 相同性解析による交差性が予測される生物種 |
| Full Name | Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) |
| Calculated molecular weight | 666 aa, 75 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC018147 |
| Gene Symbol | LEO1 |
| Gene ID (NCBI) | 123169 |
| RRID | AB_3085270 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8WVC0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LEO1 antibody 68568-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |