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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Leptin Recombinant antibody, PBS Only

Leptin Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240720A6

Cat no : 84072-5-PBS

Synonyms

LEP, 240720A6, OBS, Obesity factor, Obese protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using Leptin Recombinant antibody (84072-5-RR)

    IF Staining of 3T3-L1 using 84072-5-RR (same clone as 84072-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using LEP antibody (84072-5-RR, Clone: 240720A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Leptin Recombinant antibody (84072-5-RR)

    FC experiment of HeLa using 84072-5-RR (same clone as 84072-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Leptin Recombinant antibody (84072-5-RR)

    FC experiment of HepG2 using 84072-5-RR (same clone as 84072-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84072-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84072-5-RR

    Affinity and Kinetic Characterization of 84072-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84072-5-RR against Human LEP were performed. The affinity constant is 35.3 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84072-5-PBS is Leptin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name leptin
Calculated molecular weight 167 aa, 19 kDa
GenBank accession numberBC060830
Gene symbol Leptin
Gene ID (NCBI) 3952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.