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  • KD/KO Validated

P3H2 Polyclonal antibody

P3H2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15723-1-AP

Synonyms

leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2


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Tested Applications

Positive WB detected inL02 cells, HEK-293 cells, mouse placenta tissue, mouse kidney tissue, human placenta tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15723-1-AP targets P3H2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P3H2 fusion protein Ag8444 相同性解析による交差性が予測される生物種
Full Name leprecan-like 1
Calculated molecular weight708aa,81 kDa; 527aa,60 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC005029
Gene symbol LEPREL1
Gene ID (NCBI) 55214
RRIDAB_2136486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

Protocols

Product Specific Protocols
WB protocol for P3H2 antibody 15723-1-APDownload protocol
IHC protocol for P3H2 antibody 15723-1-APDownload protocol
IP protocol for P3H2 antibody 15723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations*

Authors - David M Hudson
humanWB

Sci Rep

Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure

Authors - Takahiro Isono
humanWB

J Biol Chem

Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.

Authors - Nathan T Montgomery
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

We conducted a screening of basal expression levels in multiple human tumor cell lines, attempting antibody concentrations ranging from 1:500 to 2000, all of which exhibited clear bands, indicating stable working conditions. However, the expression depends on the cancer cell line itself.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Multiple cancer cell lines
P3H2 Antibody Western Blot validation (1:500 dilution) in Multiple cancer cell lines (Cat no:15723-1-AP)