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- KD/KO Validated
P3H2 Polyclonal antibody
P3H2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15723-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, mouse placenta tissue, mouse kidney tissue, human placenta tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
15723-1-AP targets P3H2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | P3H2 fusion protein Ag8444 相同性解析による交差性が予測される生物種 |
| Full Name | leprecan-like 1 |
| Calculated molecular weight | 708aa,81 kDa; 527aa,60 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC005029 |
| Gene symbol | LEPREL1 |
| Gene ID (NCBI) | 55214 |
| RRID | AB_2136486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P3H2 antibody 15723-1-AP | Download protocol |
| IHC protocol for P3H2 antibody 15723-1-AP | Download protocol |
| IP protocol for P3H2 antibody 15723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations* | ||
Sci Rep Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure | ||
J Biol Chem Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shinford (Verified Customer) (10-26-2023) | We conducted a screening of basal expression levels in multiple human tumor cell lines, attempting antibody concentrations ranging from 1:500 to 2000, all of which exhibited clear bands, indicating stable working conditions. However, the expression depends on the cancer cell line itself.
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