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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LEPREL2 Monoclonal antibody

LEPREL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6F3

Cat no : 68299-1-Ig

Synonyms

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LEPREL2 Monoclonal antibody (68299-1-Ig)

    WB analysis of HeLa using 68299-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using LEPREL2 Monoclonal antibody (68299-1-Ig)

    WB analysis of rat testis using 68299-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using LEPREL2 Monoclonal antibody (68299-1-Ig)

    IHC staining of mouse ovary using 68299-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat testis tissue, HeLa cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68299-1-Ig targets LEPREL2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LEPREL2 fusion protein Ag8850 相同性解析による交差性が予測される生物種
Full Name leprecan-like 2
Calculated molecular weight 736 aa, 82 kDa
Observed molecular weight82 kDa
GenBank accession numberBC017217
Gene symbol LEPREL2
Gene ID (NCBI) 10536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.

Protocols

Product Specific Protocols
WB protocol for LEPREL2 antibody 68299-1-IgDownload protocol
IHC protocol for LEPREL2 antibody 68299-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols