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- KD/KO Validated
LETM1 Polyclonal antibody
LETM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16024-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells |
| Positive IHC detected in | human breast cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16024-1-AP targets LETM1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LETM1 fusion protein Ag8861 相同性解析による交差性が予測される生物種 |
| Full Name | leucine zipper-EF-hand containing transmembrane protein 1 |
| Calculated molecular weight | 739 aa, 83 kDa |
| Observed molecular weight | 83 kDa |
| GenBank accession number | BC021208 |
| Gene symbol | LETM1 |
| Gene ID (NCBI) | 3954 |
| RRID | AB_2136505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LETM1 antibody 16024-1-AP | Download protocol |
| IHC protocol for LETM1 antibody 16024-1-AP | Download protocol |
| IF protocol for LETM1 antibody 16024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability. | ||
Sci Adv A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance. | ||
Nat Commun Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. | ||
Proc Natl Acad Sci U S A Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.
| ||
Cell Rep The Mitochondrial Calcium Uniporter Matches Energetic Supply with Cardiac Workload during Stress and Modulates Permeability Transition. | ||
Genes Dis Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SITING (Verified Customer) (08-31-2020) | easy to expose
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