Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, rat heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | rat adrenal gland tissue, mouse adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68208-1-Ig targets LETM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | LETM1 fusion protein Ag8851 相同性解析による交差性が予測される生物種 |
| Full Name | leucine zipper-EF-hand containing transmembrane protein 1 |
| Calculated molecular weight | 739 aa, 83 kDa |
| Observed molecular weight | 83 kDa |
| GenBank accession number | BC021208 |
| Gene Symbol | LETM1 |
| Gene ID (NCBI) | 3954 |
| RRID | AB_2935296 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95202 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LETM1 antibody 68208-1-Ig | Download protocol |
| IHC protocol for LETM1 antibody 68208-1-Ig | Download protocol |
| IF protocol for LETM1 antibody 68208-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |